Hotspot

rns-trnI(gau)

IRb · 11715-11788 · 74 bp

Back to Coscinodiscus

Pi 0.234

MarkerSeek score 81.40

Primer available yes

Label rns-trnI(gau)

Feature Metadata

label_name: rns-trnI(gau)
feature_type: igs
region: IRb
length_bp: 74
variable_sites: 23
pi: 0.234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gau)_p1	GTAACCGTACTGGAAGGTGC	ATTTGAACCAGAGACCTCGC	55.0	50.0	58.3	57.9	162-175 bp	1.0	88.9
rns-trnI(gau)_p2	AGGTAACCGTACTGGAAGGT	ATTTGAACCAGAGACCTCGC	50.0	50.0	57.7	57.9	164-177 bp	1.0	88.7
rns-trnI(gau)_p3	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.3	57.9	176-189 bp	1.0	88.6
rns-trnI(gau)_p4	AGGTAACCGTACTGGAAGGT	CTGGGCCATTCTGGATTTGA	50.0	50.0	57.7	57.9	178-191 bp	1.0	88.3
rns-trnI(gau)_p5	GGTAACCGTACTGGAAGGTG	ATTTGAACCAGAGACCTCGC	55.0	50.0	57.6	57.9	163-176 bp	1.0	88.2