Hotspot

rpl20-acpP

IRb · 6915-8584 · 1670 bp

Back to Coscinodiscus

Pi 0.17

MarkerSeek score 81.30

Primer available yes

Label rpl20-acpP

Feature Metadata

label_name: rpl20-acpP
feature_type: igs
region: IRb
length_bp: 1670
variable_sites: 393
pi: 0.17
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-acpP_p1	ACAAGCCTGTCCTGTAGAGT	AGGATTTGAACCTACGGCAC	50.0	50.0	58.0	57.9	2818 bp	0.333333	52.3
rpl20-acpP_p2	GCACGCTTTCAAAGGTCATC	AGGATTTGAACCTACGGCAC	50.0	50.0	58.0	57.9	2619 bp	0.333333	52.2
rpl20-acpP_p3	CCTGTCCTGTAGAGTGGGAA	AGGATTTGAACCTACGGCAC	55.0	50.0	58.1	57.9	2813 bp	0.333333	52.0
rpl20-acpP_p4	GAAACGTAGCACGAAAACGG	AGGATTTGAACCTACGGCAC	50.0	50.0	58.1	57.9	2474 bp	0.333333	51.9
rpl20-acpP_p5	GGAAACGTAGCACGAAAACG	AGGATTTGAACCTACGGCAC	50.0	50.0	58.1	57.9	2475 bp	0.333333	51.9