Hotspot

ycf1

SSC · 124519-129990 · 5472 bp

Back to Corynonema

Pi 0.0386

MarkerSeek score 65.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 209
pi: 0.0386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTTGTATGACCACCGGGG	TATCCGCCGAAGAAGATCCT	50.0	50.0	57.9	58.0	991-1036 bp	1.0	86.3
ycf1_p2	AAGAGGGGAATGGGCATTTG	TATCCGCCGAAGAAGATCCT	50.0	50.0	58.1	58.0	1238-1283 bp	1.0	85.9
ycf1_p3	GCATTTGCTTGAACCGGTTT	TATCCGCCGAAGAAGATCCT	45.0	50.0	58.1	58.0	1225-1270 bp	1.0	85.9
ycf1_p4	TGCGCGTATGGAACCTTTAA	TATCCGCCGAAGAAGATCCT	45.0	50.0	57.9	58.0	1226 bp	0.5	59.2
ycf1_p5	AACCGTTTTGCGTCTTTGTG	TATCCGCCGAAGAAGATCCT	45.0	50.0	58.1	58.0	1244 bp	0.5	59.2