Hotspot

ndhD

SSC · 116245-117723 · 1479 bp

Back to Corynonema

Pi 0.027

MarkerSeek score 60.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1479
variable_sites: 40
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAATTGTCGCTTCTACGGGA	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2102-2191 bp	1.0	86.3
ndhD_p2	CAATTGTCGCTTCTACGGGA	GTGTTGGTTGTAAGCGATGC	50.0	50.0	58.0	58.0	2080-2169 bp	1.0	86.2
ndhD_p3	CGTGGGGTTCGTATTGGAAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2219-2308 bp	1.0	85.8
ndhD_p4	CGTGGGGTTCGTATTGGAAT	GTGTTGGTTGTAAGCGATGC	50.0	50.0	58.0	58.0	2197-2286 bp	1.0	85.7
ndhD_p5	AAATGAGCCGTTTTGCTTCG	TCTGCTCCAAGAACAGAGGA	45.0	50.0	57.9	58.0	2416-2505 bp	1.0	85.4