Hotspot

rpl32-trnL-UAG

SSC · 114211-114816 · 606 bp

Back to Corynonema

Pi 0.0475

MarkerSeek score 75.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 606
variable_sites: 26
pi: 0.0475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAATCATAGGCCGGTCCAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1076 bp	0.5	59.0
rpl32-trnL-UAG_p2	GAAATCATAGGCCGGTCCAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1077 bp	0.5	59.0
rpl32-trnL-UAG_p3	CCAGAGCGTTCCCCAATAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1060 bp	0.5	59.0
rpl32-trnL-UAG_p4	CCAGAGCGTTCCCCAATAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1061 bp	0.5	58.9
rpl32-trnL-UAG_p5	GAAATCATAGGCCGGTCCAG	CGAAGCAAAACGGCTCATTT	55.0	45.0	58.1	57.9	1787 bp	0.5	58.7