Hotspot

trnF-GAA-ndhJ

LSC · 50062-50536 · 475 bp

Back to Corynonema

Pi 0.0582

MarkerSeek score 77.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 23
pi: 0.0582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	555-623 bp	1.0	86.8
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	554-622 bp	1.0	86.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	519-587 bp	1.0	86.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	518-586 bp	1.0	86.6
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.9	58.0	542-610 bp	1.0	85.8