Hotspot

trnE-UUC-trnT-GGU

LSC · 32294-33023 · 730 bp

Back to Corynonema

Pi 0.0528

MarkerSeek score 74.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 38
pi: 0.0528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ATTGAACTACAATCCCGGGC	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1479-1570 bp	1.0	85.1
trnE-UUC-trnT-GGU_p2	ATTGAACTACAATCCCGGGC	ATATCGACGGATTCTTGCGG	50.0	50.0	57.9	57.9	1965-2071 bp	1.0	83.9
trnE-UUC-trnT-GGU_p3	TGGATCGGCGAATTATTGGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1017-1138 bp	1.0	83.0
trnE-UUC-trnT-GGU_p4	TTCGTATCCGGCATTTCTGG	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1066 bp	0.5	59.5
trnE-UUC-trnT-GGU_p5	TTCGTATCCGGCATTTCTGG	ATATCGACGGATTCTTGCGG	50.0	50.0	58.1	57.9	1552 bp	0.5	59.1