Hotspot

rpoB-trnC-GCA

LSC · 27368-28611 · 1244 bp

Back to Corynonema

Pi 0.0495
MarkerSeek score 83.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1244
variable_sites
58
pi
0.0495
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCGCATTTCCATCTCC ATCGTTTGTTGATCAGGCGA 50.0 45.0 58.0 57.9 1306-1355 bp 1.0 86.6
rpoB-trnC-GCA_p2 ATTCCCGCATTTCCATCTCC GTTTGTTGATCAGGCGACAC 50.0 50.0 58.0 57.7 1303-1352 bp 1.0 84.9
rpoB-trnC-GCA_p3 ATTCCCGCATTTCCATCTCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1265-1314 bp 1.0 84.6
rpoB-trnC-GCA_p4 ATTCCCGCATTTCCATCTCC GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1290-1339 bp 1.0 83.6
rpoB-trnC-GCA_p5 GCATTTCCATCTCCGAGCAT ATCGTTTGTTGATCAGGCGA 50.0 45.0 58.4 57.9 1300-1349 bp 1.0 83.6