Hotspot

trnS-GCU-trnG-UCC

LSC · 8210-9091 · 882 bp

Back to Corynonema

Pi 0.0631

MarkerSeek score 76.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 44
pi: 0.0631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	786-950 bp	1.0	60.7
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	783-947 bp	1.0	59.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.6	844-1008 bp	1.0	59.3
trnS-GCU-trnG-UCC_p4	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	785-949 bp	1.0	58.7
trnS-GCU-trnG-UCC_p5	ACGCTTTAATCCACTCAGCC	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.3	57.6	783-947 bp	1.0	58.7