Hotspot

rps16-trnQ-UUG

LSC · 6103-6893 · 791 bp

Back to Corynonema

Pi 0.0499

MarkerSeek score 75.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 39
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCGGATCATGTCCTTCAA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1393-1456 bp	1.0	86.6
rps16-trnQ-UUG_p2	CGGGATACGCTTTTGATGGA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	1713-1776 bp	1.0	86.2
rps16-trnQ-UUG_p3	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	882-888 bp	1.0	83.7
rps16-trnQ-UUG_p4	CAGCGGATCATGTCCTTCAA	GAAATTGAAATGGGGCGTCG	50.0	50.0	58.0	58.1	940-946 bp	1.0	83.4
rps16-trnQ-UUG_p5	CGGGATACGCTTTTGATGGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1202-1208 bp	1.0	83.3