Hotspot

trnH-GUG-psbA

LSC · 100-407 · 308 bp

Back to Corynonema

Pi 0.0779

MarkerSeek score 79.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 24
pi: 0.0779
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGTGCTAACCTTGGTATGGA	55.0	50.0	58.1	57.3	426-516 bp	1.0	77.6
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCGTGCTAACCTTGGTATGG	55.0	50.0	58.1	57.3	427-517 bp	1.0	77.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	440-530 bp	1.0	77.4
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TCGTGCTAACCTTGGTATGG	50.0	50.0	57.9	57.3	441-531 bp	1.0	77.4
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	439-529 bp	1.0	77.4