Hotspot

ndhF

SSC · 114995-117247 · 2253 bp

Back to Corymbia

Pi 0.0024

MarkerSeek score 46.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 13
pi: 0.0024
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGATCCGGACAAAATCGA	TTTTATTGCGCCCGTTTTGG	50.0	45.0	57.4	58.2	2969-2976 bp	1.0	74.4
ndhF_p2	GAGGATCCGGACAAAATCGA	TATTGCGCCCGTTTTGGTAT	50.0	45.0	57.4	58.2	2966-2973 bp	1.0	74.2
ndhF_p3	GAGGATCCGGACAAAATCGA	TTATTGCGCCCGTTTTGGTA	50.0	45.0	57.4	58.5	2967-2974 bp	1.0	72.4
ndhF_p4	GGAAGAGATCCGAGTGAATGG	TTTTATTGCGCCCGTTTTGG	52.4	45.0	57.9	58.2	2943-2950 bp	1.0	70.4
ndhF_p5	GAGGATCCGGACAAAATCGAT	TTTTATTGCGCCCGTTTTGG	47.6	45.0	57.9	58.2	2969-2976 bp	1.0	70.3