Hotspot

rpl32-trnL-UAG

SSC · 118343-119107 · 765 bp

Back to Corymbia

Pi 0.00732

MarkerSeek score 52.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 765
variable_sites: 13
pi: 0.00732
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGTATATTGGGCAGCGTTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	867-882 bp	1.0	82.6
rpl32-trnL-UAG_p2	AGGTATATTGGGCAGCGTTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	868-883 bp	1.0	82.6
rpl32-trnL-UAG_p3	GGTATATTGGGCAGCGTTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	866-881 bp	1.0	82.5
rpl32-trnL-UAG_p4	GGTATATTGGGCAGCGTTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	867-882 bp	1.0	82.5
rpl32-trnL-UAG_p5	AGGTATATTGGGCAGCGTTG	TTGGGATTGGAGAGCAGGTA	50.0	50.0	58.0	58.0	1433-1448 bp	1.0	81.3