Hotspot

ndhF-rpl32

SSC · 117248-118189 · 942 bp

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Pi 0.00538

MarkerSeek score 54.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 942
variable_sites: 12
pi: 0.00538
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAGGGG	CAACGCTGCCCAATATACCT	55.0	50.0	57.9	58.0	1075-1083 bp	1.0	80.1
ndhF-rpl32_p2	CTGGAAGTGGAATGAAGGGG	TCAACGCTGCCCAATATACC	55.0	50.0	57.9	58.0	1076-1084 bp	1.0	80.1
ndhF-rpl32_p3	TGGAAGTGGAATGAAGGGGA	CAACGCTGCCCAATATACCT	50.0	50.0	58.2	58.0	1074-1082 bp	1.0	79.3
ndhF-rpl32_p4	TGGAAGTGGAATGAAGGGGA	TCAACGCTGCCCAATATACC	50.0	50.0	58.2	58.0	1075-1083 bp	1.0	79.2
ndhF-rpl32_p5	CTGGAAGTGGAATGAAGGGG	ACGCTGCCCAATATACCTTC	55.0	50.0	57.9	57.7	1073-1081 bp	1.0	78.4