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psbE-petL

LSC · 68784-70050 · 1267 bp

Back to Corymbia

Pi 0.000947

MarkerSeek score 51.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 3
pi: 0.000947
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.9	56.4	1459-1464 bp	1.0	66.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1412-1417 bp	1.0	66.1
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.8	56.4	1382-1387 bp	1.0	66.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGGTCAGACC	45.0	50.0	58.2	56.4	1468-1473 bp	1.0	65.9
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGTCGTATCTTGGTCAGACC	50.0	50.0	58.2	56.4	1473-1478 bp	1.0	65.8