Hotspot

atpB-rbcL

LSC · 57967-58764 · 798 bp

Back to Corymbia

Pi 0.00192

MarkerSeek score 51.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 4
pi: 0.00192
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AAACTCCAGGACCCGAAGTA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	883-884 bp	1.0	72.2
atpB-rbcL_p2	AAACTCCAGGACCCGAAGTA	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	882-883 bp	1.0	71.9
atpB-rbcL_p3	GATTTGAGCGATACGTCCCA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	924-925 bp	1.0	70.5
atpB-rbcL_p4	TGATTTGAGCGATACGTCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	925-926 bp	1.0	70.5
atpB-rbcL_p5	ATTTGAGCGATACGTCCCAG	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	923-924 bp	1.0	70.5