Hotspot

ndhC-trnV-UAC

LSC · 54567-54884 · 318 bp

Back to Corymbia

Pi 0.0113

MarkerSeek score 54.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 318
variable_sites: 7
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATCGGGGCTAAAATTCCGG	TGGGTCTTTGAACCAGTTCG	50.0	50.0	58.3	58.0	511-721 bp	1.0	82.0
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	TGGGTCTTTGAACCAGTTCG	50.0	50.0	57.7	58.0	519-729 bp	1.0	81.7
ndhC-trnV-UAC_p3	AATCGGGGCTAAAATTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	453-663 bp	1.0	80.9
ndhC-trnV-UAC_p4	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	461-671 bp	1.0	80.7
ndhC-trnV-UAC_p5	AATCGGGGCTAAAATTCCGG	GGGTCTTTGAACCAGTTCGA	50.0	50.0	58.3	57.8	510-720 bp	1.0	80.4