Hotspot

psaA-ycf3

LSC · 45044-45926 · 883 bp

Back to Corymbia

Pi 0.00257

MarkerSeek score 56.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 5
pi: 0.00257
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	1037-1081 bp	1.0	82.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1018-1062 bp	1.0	82.5
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	979-1023 bp	1.0	82.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	960-1004 bp	1.0	82.2
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	1039-1083 bp	1.0	81.8