Hotspot

psbZ-trnG-UCC

LSC · 38694-39659 · 966 bp

Back to Corymbia

Pi 0.00612

MarkerSeek score 61.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 14
pi: 0.00612
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	1024-1112 bp	1.0	82.6
psbZ-trnG-UCC_p2	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	1036-1124 bp	1.0	80.4
psbZ-trnG-UCC_p3	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	1037-1125 bp	1.0	79.1
psbZ-trnG-UCC_p4	ACGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	1023-1111 bp	1.0	78.9
psbZ-trnG-UCC_p5	GACGGTTGGTCGAGTAACAA	ACTTTAACCTGGGCGGATAG	50.0	50.0	57.9	57.0	1059-1147 bp	1.0	75.9