Hotspot

trnS-UGA-psbZ

LSC · 38158-38504 · 347 bp

Back to Corymbia

Pi 0.00442
MarkerSeek score 45.70
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
347
variable_sites
4
pi
0.00442
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 TTCACCGAGCAAAAGGAGAG TTGTTACTCGACCAACCGTC 50.0 50.0 57.8 57.9 563 bp 1.0 78.1
trnS-UGA-psbZ_p2 TTTTCAAGACCGGAGCCATC TTGTTACTCGACCAACCGTC 50.0 50.0 58.2 57.9 497 bp 1.0 78.1
trnS-UGA-psbZ_p3 CACCGAGCAAAAGGAGAGAG TTGTTACTCGACCAACCGTC 55.0 50.0 58.3 57.9 561 bp 1.0 77.2
trnS-UGA-psbZ_p4 CCGAGCAAAAGGAGAGAGAG TTGTTACTCGACCAACCGTC 55.0 50.0 57.7 57.9 559 bp 1.0 77.1
trnS-UGA-psbZ_p5 CGAGCAAAAGGAGAGAGAGG TTGTTACTCGACCAACCGTC 55.0 50.0 57.7 57.9 558 bp 1.0 77.1