Hotspot

trnC-GCA-petN

LSC · 29640-30521 · 882 bp

Back to Corymbia

Pi 0.00189

MarkerSeek score 51.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 5
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	944-945 bp	1.0	79.9
trnC-GCA-petN_p2	CATACATTTTGGCGGCATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	996-997 bp	1.0	79.4
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	938-939 bp	1.0	78.4
trnC-GCA-petN_p4	CATACATTTTGGCGGCATGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	990-991 bp	1.0	77.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	962-963 bp	1.0	77.7