Hotspot

rpoB-trnC-GCA

LSC · 28315-29568 · 1254 bp

Back to Corymbia

Pi 0.00292

MarkerSeek score 54.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 8
pi: 0.00292
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATCATTTCCATCCCCG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.1	58.1	1341 bp	1.0	79.0
rpoB-trnC-GCA_p2	CCTCATCATTTCCATCCCCG	TTTCTTGATCAGGCGACACC	55.0	50.0	58.1	58.2	1360 bp	1.0	78.2
rpoB-trnC-GCA_p3	CCCTCATCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.7	58.1	1342 bp	1.0	77.4
rpoB-trnC-GCA_p4	CCTCATCATTTCCATCCCCG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.1	58.4	1323 bp	1.0	76.9
rpoB-trnC-GCA_p5	CCCTCATCATTTCCATCCCC	TTTCTTGATCAGGCGACACC	55.0	50.0	57.7	58.2	1361 bp	1.0	76.5