Hotspot

trnR-UCU-atpA

LSC · 10865-11204 · 340 bp

Back to Corymbia

Pi 0.0098

MarkerSeek score 61.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 8
pi: 0.0098
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	436-439 bp	1.0	67.6
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CAGGAACAGAAGGAACGCTT	47.6	50.0	57.3	58.1	403-406 bp	1.0	67.5
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	435-438 bp	1.0	65.3
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	434-437 bp	1.0	65.3
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	56.9	58.1	471-474 bp	1.0	64.6