Hotspot

trnS-GCU-trnR-UCU

LSC · 8920-10792 · 1873 bp

Back to Corymbia

Pi 0.00303
MarkerSeek score 48.30
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1873
variable_sites
14
pi
0.00303
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1920-1944 bp 1.0 65.2
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1919-1943 bp 1.0 65.2
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1926-1950 bp 1.0 64.5
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1925-1949 bp 1.0 64.5
trnS-GCU-trnR-UCU_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1974-1998 bp 1.0 64.0