Hotspot

ycf1

SSC · 129300-135038 · 5739 bp

Back to Corylus

Pi 0.003

MarkerSeek score 41.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5739
variable_sites: 63
pi: 0.003
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTTGGCCCATTTTTGTCAT	AGTTTGCAGGAAAATCAACATCA	45.0	34.8	57.5	57.5	266 bp	1.0	49.9
ycf1_p2	TTCGTTGGCCCATTTTTGTC	AGTTTGCAGGAAAATCAACATCA	45.0	34.8	57.5	57.5	268 bp	1.0	49.4
ycf1_p3	TCGTTGGCCCATTTTTGTCA	AGTTTGCAGGAAAATCAACATCA	45.0	34.8	58.6	57.5	267 bp	1.0	48.8
ycf1_p4	CGTTGGCCCATTTTTGTCAT	GTTTGCAGGAAAATCAACATCA	45.0	36.4	57.5	56.1	265 bp	1.0	46.7
ycf1_p5	TTCGTTGGCCCATTTTTGTC	GTTTGCAGGAAAATCAACATCA	45.0	36.4	57.5	56.1	267 bp	1.0	46.2