Hotspot

ndhF

SSC · 115077-117317 · 2241 bp

Back to Corylus

Pi 0.0166

MarkerSeek score 60.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 171
pi: 0.0166
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGGAATCGCCCATTTCGAT	TGTCCTTACTCTCTCCCGAG	45.0	55.0	57.7	57.6	2348-2798 bp	1.0	84.8
ndhF_p2	GATGGAATCGCCCATTTCGA	TGTCCTTACTCTCTCCCGAG	50.0	55.0	58.4	57.6	2349-2799 bp	1.0	83.7
ndhF_p3	ATGGAATCGCCCATTTCGAT	GTCCTTACTCTCTCCCGAGA	45.0	55.0	57.7	57.3	2347-2797 bp	1.0	82.4
ndhF_p4	GATGGAATCGCCCATTTCGA	GTCCTTACTCTCTCCCGAGA	50.0	55.0	58.4	57.3	2348-2798 bp	1.0	81.4
ndhF_p5	ATGGAATCGCCCATTTCGAT	TTGTCCTTACTCTCTCCCGA	45.0	50.0	57.7	57.1	2349-2799 bp	1.0	81.0