Hotspot

accD

LSC · 61652-63154 · 1503 bp

Back to Corylus

Pi 0.00229

MarkerSeek score 30.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 13
pi: 0.00229
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTTTTCGTTCTGTGTTGGGC	TCGTTTTATTCGCCTTGCCT	50.0	45.0	57.9	58.2	2174-2183 bp	1.0	79.1
accD_p2	CCCGTAGTTTCCGATCATGG	TCGTTTTATTCGCCTTGCCT	55.0	45.0	58.1	58.2	2240-2249 bp	1.0	78.9
accD_p3	ATTGTTGTGTTGGGTCCACA	TCGTTTTATTCGCCTTGCCT	45.0	45.0	57.8	58.2	2302-2311 bp	1.0	78.7
accD_p4	CGTTCTGTGTTGGGCTAGAA	TCGTTTTATTCGCCTTGCCT	50.0	45.0	57.8	58.2	2169-2178 bp	1.0	78.7
accD_p5	CGGCGGGATCACTATTTGAA	TCGTTTTATTCGCCTTGCCT	50.0	45.0	58.1	58.2	2043-2045 bp	0.222222	47.5