Hotspot

trnL-TAG-ccsA

SSC · 119512-119624 · 113 bp

Back to Corylus

Pi 0.0141
MarkerSeek score 48.70
Primer available yes
Label trnL-TAG-ccsA

Feature Metadata

label_name
trnL-TAG-ccsA
feature_type
igs
region
SSC
length_bp
113
variable_sites
6
pi
0.0141
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.96
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ACAACCGAAAAGGATATATGAGT 50.0 34.8 57.9 55.5 221-229 bp 1.0 44.3
trnL-TAG-ccsA_p2 GTGAAATCGGTAGACACGCT ACAACCGAAAAGGATATATGAGT 50.0 34.8 58.0 55.5 231-239 bp 1.0 44.1
trnL-TAG-ccsA_p3 TGAAATCGGTAGACACGCTG ACAACCGAAAAGGATATATGAGT 50.0 34.8 58.0 55.5 230-238 bp 1.0 44.1
trnL-TAG-ccsA_p4 AACAAGCCGCTATGGTGAAA ACAACCGAAAAGGATATATGAGT 45.0 34.8 58.1 55.5 245-253 bp 1.0 43.9
trnL-TAG-ccsA_p5 TGATTTCGAACAAGCCGCTA ACAACCGAAAAGGATATATGAGT 45.0 34.8 57.9 55.5 253-261 bp 1.0 43.8