Hotspot

rpl32-trnL-TAG

SSC · 118385-119431 · 1047 bp

Back to Corylus

Pi 0.00687
MarkerSeek score 50.30
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
1047
variable_sites
24
pi
0.00687
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.95
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 GGAGGGGTATTGGGTAGCAT AGCGTGTCTACCGATTTCAC 55.0 50.0 58.6 58.0 681-1175 bp 1.0 81.2
rpl32-trnL-TAG_p2 GGAGGGGTATTGGGTAGCAT CAGCGTGTCTACCGATTTCA 55.0 50.0 58.6 58.0 682-1176 bp 1.0 81.2
rpl32-trnL-TAG_p3 GGAGGGGTATTGGGTAGCAT TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.6 57.9 691-1185 bp 1.0 80.3
rpl32-trnL-TAG_p4 GGAGGGGTATTGGGTAGCAT CCTAAGAGCAGCGTGTCTAC 55.0 55.0 58.6 57.8 690-1184 bp 1.0 79.7
rpl32-trnL-TAG_p5 GGAGGGGTATTGGGTAGCAT CTAAGAGCAGCGTGTCTACC 55.0 55.0 58.6 57.8 689-1183 bp 1.0 79.7