Hotspot

trnP-TGG-psaJ

LSC · 71163-71559 · 397 bp

Back to Corylus

Pi 0.00861

MarkerSeek score 42.50

Primer available yes

Label trnP-TGG-psaJ

Feature Metadata

label_name: trnP-TGG-psaJ
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 13
pi: 0.00861
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-TGG-psaJ_p1	ATCCCTCGTTACGGCTCATA	GCCGCGGAGAGATATGTTTT	50.0	50.0	58.0	58.4	537-554 bp	1.0	78.8
trnP-TGG-psaJ_p2	ATCCCTCGTTACGGCTCATA	TCAACGCATCCGGGAATAAA	50.0	45.0	58.0	57.6	617-634 bp	1.0	78.6
trnP-TGG-psaJ_p3	ATCCCTCGTTACGGCTCATA	CAACGCATCCGGGAATAAAC	50.0	50.0	58.0	57.5	616-633 bp	1.0	78.1
trnP-TGG-psaJ_p4	ACCCGTGACATTTTGTACCC	TCAACGCATCCGGGAATAAA	50.0	45.0	58.1	57.6	564-581 bp	1.0	78.1
trnP-TGG-psaJ_p5	ATCCCTCGTTACGGCTCATA	CGCATCCGGGAATAAACGAT	50.0	50.0	58.0	58.5	613-630 bp	1.0	77.9