Hotspot

psbL-psbF

LSC · 68916-68937 · 22 bp

Back to Corylus

Pi 0.0214

MarkerSeek score 49.10

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 1
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	GGTTGGCTGTTCACGGATTA	45.0	50.0	58.0	58.2	125 bp	1.0	78.8
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	GCTGTTCACGGATTAGCTGT	45.0	50.0	58.0	58.3	120 bp	1.0	78.1
psbL-psbF_p3	CGTTCGGGTTTGATTGTGTC	GGTTGGCTGTTCACGGATTA	50.0	50.0	57.7	58.2	124 bp	1.0	76.7
psbL-psbF_p4	CGTTCGGGTTTGATTGTGTC	GCTGTTCACGGATTAGCTGT	50.0	50.0	57.7	58.3	119 bp	1.0	76.0
psbL-psbF_p5	TCGTTCGGGTTTGATTGTGT	TTGGCTGTTCACGGATTAGC	45.0	50.0	58.0	58.6	123 bp	1.0	75.9