Hotspot

trnF-GAA-ndhJ

LSC · 52498-53323 · 826 bp

Back to Corylus

Pi 0.00382

MarkerSeek score 56.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 12
pi: 0.00382
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	938-982 bp	1.0	82.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	902-946 bp	1.0	81.8
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	926-970 bp	1.0	81.1
trnF-GAA-ndhJ_p4	AAGATAAGGATGATGCGCCG	ATGCCGGAAAGTTGGATAGG	50.0	50.0	58.2	57.7	967-1011 bp	1.0	80.6
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCGGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	936-980 bp	1.0	79.8