Hotspot

petN-psbM

LSC · 31446-32399 · 954 bp

Back to Corylus

Pi 0.00303

MarkerSeek score 49.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 12
pi: 0.00303
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1103-1125 bp	1.0	70.0
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1103-1125 bp	1.0	65.8
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1104-1126 bp	1.0	64.0
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.9	57.6	1095-1117 bp	1.0	64.0
petN-psbM_p5	ATGGGGTAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.0	57.6	1042-1064 bp	1.0	63.7