Hotspot

rpoB-trnC-GCA

LSC · 29147-30409 · 1263 bp

Back to Corylus

Pi 0.00347

MarkerSeek score 58.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 13
pi: 0.00347
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTCCCTCATTTCCACCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.2	58.4	1277-1338 bp	1.0	80.0
rpoB-trnC-GCA_p2	CATTCCCTCATTTCCACCCC	GCGACACCCAGATTTGAACT	55.0	50.0	58.2	58.5	1302-1363 bp	1.0	78.9
rpoB-trnC-GCA_p3	CATTCCCTCATTTCCACCCC	ATTGTTGATCAGGCGACACC	55.0	50.0	58.2	58.5	1314-1375 bp	1.0	78.3
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCACCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.5	58.4	1278-1339 bp	1.0	77.5
rpoB-trnC-GCA_p5	ACATTCCCTCATTTCCACCC	GCGACACCCAGATTTGAACT	50.0	50.0	57.5	58.5	1303-1364 bp	1.0	76.4