Hotspot

atpH-atpI

LSC · 15566-16787 · 1222 bp

Back to Corylus

Pi 0.00444

MarkerSeek score 57.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 19
pi: 0.00444
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1259-1309 bp	1.0	83.8
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1344-1394 bp	1.0	83.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1260-1310 bp	1.0	82.6
atpH-atpI_p4	CGATCCCTTCTACAGCTTGG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.8	57.9	1345-1395 bp	1.0	82.2
atpH-atpI_p5	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	1292-1342 bp	1.0	81.7