Hotspot

trnG-UCC-trnR-TCT

LSC · 11251-11548 · 298 bp

Back to Corylus

Pi 0.0111

MarkerSeek score 55.40

Primer available yes

Label trnG-UCC-trnR-TCT

Feature Metadata

label_name: trnG-UCC-trnR-TCT
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 15
pi: 0.0111
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-TCT_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	362-388 bp	1.0	70.5
trnG-UCC-trnR-TCT_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	365-391 bp	1.0	70.3
trnG-UCC-trnR-TCT_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	368-394 bp	1.0	69.8
trnG-UCC-trnR-TCT_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	371-397 bp	1.0	69.6
trnG-UCC-trnR-TCT_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	432-458 bp	1.0	68.2