Hotspot

matK-rps16

LSC · 3665-5534 · 1870 bp

Back to Corylus

Pi 0.00308

MarkerSeek score 51.90

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1870
variable_sites: 23
pi: 0.00308
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TGGGTATAGGAAGTCATGTTGC	AGGCGGGAGTTTTTATGGAC	45.5	50.0	57.8	57.9	1845-1984 bp	1.0	66.7
matK-rps16_p2	TGGGTATAGGAAGTCATGTTGC	GCGGGAGTTTTTATGGACCT	45.5	50.0	57.8	57.9	1843-1982 bp	1.0	66.7
matK-rps16_p3	TGGGTATAGGAAGTCATGTTGC	ACATTTCAAAGAAGGCGGGA	45.5	45.0	57.8	57.7	1857-1996 bp	1.0	65.4
matK-rps16_p4	TGGGTATAGGAAGTCATGTTGC	TCAAAGAAGGCGGGAGTTTT	45.5	45.0	57.8	57.6	1852-1991 bp	1.0	64.9
matK-rps16_p5	TGGGTATAGGAAGTCATGTTGC	TTCAAAGAAGGCGGGAGTTT	45.5	45.0	57.8	57.6	1853-1992 bp	1.0	64.9