Hotspot

trnH-GTG-psbA

LSC · 76-503 · 428 bp

Back to Corylus

Pi 0.00718
MarkerSeek score 51.70
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
428
variable_sites
11
pi
0.00718
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 538-584 bp 1.0 83.2
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 552-598 bp 1.0 83.1
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 551-597 bp 1.0 83.1
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 553-599 bp 1.0 83.0
trnH-GTG-psbA_p5 CGCATGGTGGATTCACAATC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.5 58.0 567-613 bp 1.0 80.2