Hotspot

rps8

LSC · 83546-83950 · 405 bp

Back to Corylopsis

Pi 0.00212

MarkerSeek score 29.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 6
pi: 0.00212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CAACTGCCTAATCGGTCGAA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	1003 bp	1.0	81.1
rps8_p2	CGAACCCGGAACATACCATT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.0	58.0	833 bp	1.0	81.0
rps8_p3	CAACTGCCTAATCGGTCGAA	AGGAGCTAGTAATCGCCGAT	50.0	50.0	58.0	58.1	1212 bp	1.0	80.5
rps8_p4	CAACTGCCTAATCGGTCGAA	AATAGCGGCGTCAAAAATGC	50.0	45.0	58.0	58.1	1465 bp	1.0	80.5
rps8_p5	CAACTGCCTAATCGGTCGAA	GCCCGAGAATTGAGACAGTT	50.0	50.0	58.0	57.9	980 bp	1.0	80.5