Hotspot

clpP

LSC · 73494-75521 · 2028 bp

Back to Corylopsis

Pi 0.00243

MarkerSeek score 43.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2028
variable_sites: 23
pi: 0.00243
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTTGCGTGAAATCCCTGC	TCCATTGTTACGTTTCCAAATCA	50.0	34.8	58.1	57.0	2104-2122 bp	1.0	48.5
clpP_p2	TTTGCGTGAAATCCCTGCTA	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.8	57.0	2102-2120 bp	1.0	47.6
clpP_p3	GATTTGCGTGAAATCCCTGC	ACGTTTCCAAATCAAAGTGA	50.0	35.0	58.1	53.6	2095-2113 bp	1.0	45.9
clpP_p4	TGCGTGAAATCCCTGCTATT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2100-2118 bp	1.0	45.8
clpP_p5	TTGCGTGAAATCCCTGCTAT	TCCATTGTTACGTTTCCAAATCA	45.0	34.8	57.6	57.0	2101-2119 bp	1.0	45.8