Hotspot

psbZ-trnG-GCC

LSC · 38643-39341 · 699 bp

Back to Corylopsis

Pi 0.00388

MarkerSeek score 60.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 11
pi: 0.00388
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	444-856 bp	1.0	82.3
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	442-854 bp	1.0	81.9
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	ACATTACTTTACCGTCGGCG	50.0	50.0	57.8	58.4	484-896 bp	1.0	80.7
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	ACATTACTTTACCGTCGGCG	55.0	50.0	58.3	58.4	482-894 bp	1.0	80.3
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	417-829 bp	1.0	77.8