Hotspot

trnE-UUC-trnT-GGU

LSC · 33166-34048 · 883 bp

Back to Corylopsis

Pi 0.00447

MarkerSeek score 45.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 17
pi: 0.00447
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1019-1038 bp	1.0	80.4
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	984-1003 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	981-1000 bp	1.0	79.6
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	1016-1035 bp	1.0	79.2
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	946-965 bp	1.0	78.9