Hotspot

petN-psbM

LSC · 30006-31204 · 1199 bp

Back to Corylopsis

Pi 0.00335

MarkerSeek score 54.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1199
variable_sites: 20
pi: 0.00335
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1354-1363 bp	1.0	69.8
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1293-1302 bp	1.0	68.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1354-1363 bp	1.0	65.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1293-1302 bp	1.0	64.1
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1355-1364 bp	1.0	63.8