Hotspot

ndhD

IRa · 156155-157663 · 1509 bp

Back to Corydalis

Pi 0.0522
MarkerSeek score 80.00
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
IRa
length_bp
1509
variable_sites
1043
pi
0.0522
unique_haplotype_count
36
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 CGCAAATGAAAGAGGCAGTG CCGCTATCATGAGCAGATCC 50.0 55.0 58.0 58.0 1814-2656 bp 1.0 91.7
ndhD_p2 CGCAATTGTGGCTTCTCTTG CCGCTATCATGAGCAGATCC 50.0 55.0 58.0 58.0 1529-2371 bp 1.0 91.7
ndhD_p3 CGCAAATGAAAGAGGCAGTG AATTGCTTCTGCCCCAAGAA 50.0 45.0 58.0 58.0 1557-2399 bp 0.979592 90.9
ndhD_p4 CGCAATTGTGGCTTCTCTTG AAATTGCTTCTGCCCCAAGA 50.0 45.0 58.0 58.0 1273-2115 bp 0.979592 90.9
ndhD_p5 CGCAATTGTGGCTTCTCTTG AATTGCTTCTGCCCCAAGAA 50.0 45.0 58.0 58.0 1272-2114 bp 0.979592 90.9
ndhD_p1 CCGCTATCATGAGCAGATCC CGCAAATGAAAGAGGCAGTG 55.0 50.0 58.0 58.0 1814-2656 bp 0.979592 90.9
ndhD_p2 CCGCTATCATGAGCAGATCC CGCAATTGTGGCTTCTCTTG 55.0 50.0 58.0 58.0 1529-2371 bp 0.979592 90.9
ndhD_p3 AAATTGCTTCTGCCCCAAGA CGCAATTGTGGCTTCTCTTG 45.0 50.0 58.0 58.0 1273-2115 bp 0.959184 90.0
ndhD_p4 AATTGCTTCTGCCCCAAGAA CGCAAATGAAAGAGGCAGTG 45.0 50.0 58.0 58.0 1557-2399 bp 0.959184 90.0
ndhD_p5 AATTGCTTCTGCCCCAAGAA CGCAATTGTGGCTTCTCTTG 45.0 50.0 58.0 58.0 1272-2114 bp 0.959184 90.0