Hotspot

ndhF-rpl32

IRb · 117770-120308 · 2539 bp

Back to Corydalis

Pi 0.201
MarkerSeek score 73.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
IRb
length_bp
2539
variable_sites
1967
pi
0.201
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.83
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGATCCATGCATATTGATATGT GTACGTTTCTTTGGAACCGC 31.8 50.0 53.1 57.7 1987-2739 bp 0.346939 25.9
ndhF-rpl32_p2 ATGATCCATGCATATTGATATGT GTACGTTTCTTTGGAACCGC 30.4 50.0 53.8 57.7 1988-2740 bp 0.346939 25.9
ndhF-rpl32_p3 TGCATATTGATATGTATGTTCCA GTACGTTTCTTTGGAACCGC 30.4 50.0 53.7 57.7 1980-2732 bp 0.346939 25.9
ndhF-rpl32_p4 TGATCCATGCATATTGATATGT TACGTTTCTTTGGAACCGCC 31.8 50.0 53.1 58.8 1986-2738 bp 0.346939 25.9
ndhF-rpl32_p5 ATGATCCATGCATATTGATATGT TACGTTTCTTTGGAACCGCC 30.4 50.0 53.8 58.8 1987-2739 bp 0.346939 25.9