Hotspot

psbE-petL

LSC · 67746-68975 · 1230 bp

Back to Corydalis

Pi 0.0573

MarkerSeek score 79.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 431
pi: 0.0573
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CATTGCGGATATTGACCCCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	2019-2199 bp	0.979592	90.7
psbE-petL_p2	TCGTTCGGGTTTGATTGTGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2108-2288 bp	0.836735	85.0
psbE-petL_p3	CATTGCGGATATTGACCCCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	2041-2221 bp	0.816327	84.4
psbE-petL_p4	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1871-2051 bp	0.816327	84.2
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1782-1958 bp	0.714286	80.0