Hotspot

ndhC-psaI

LSC · 59757-62022 · 2266 bp

Back to Corydalis

Pi 0.326

MarkerSeek score 73.50

Primer available yes

Label ndhC-psaI

Feature Metadata

label_name: ndhC-psaI
feature_type: igs
region: LSC
length_bp: 2266
variable_sites: 1929
pi: 0.326
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-psaI_p1	GAAACTCATCGCCCATGGAT	CGGAACTACTAGGCCCACTA	50.0	55.0	58.0	57.9	919-2963 bp	0.693878	79.0
ndhC-psaI_p2	GAAACTCATCGCCCATGGAT	CGGTTTCATACATCTGCGGA	50.0	50.0	58.0	58.1	1390-2957 bp	0.326531	64.3
ndhC-psaI_p3	GAAACTCATCGCCCATGGAT	TCGGTTTCATACATCTGCGG	50.0	50.0	58.0	58.1	1391-2958 bp	0.326531	64.3
ndhC-psaI_p4	CTGTGCCGGCTGTTAAAATG	CGGAACTACTAGGCCCACTA	50.0	55.0	58.0	57.9	1324-2991 bp	0.306122	63.6
ndhC-psaI_p5	GCTAGAAAGCTTCTCCGTCC	GTTTTTCGAGACCCCGCTAT	55.0	50.0	58.1	58.0	1529-2995 bp	0.102041	55.4