Hotspot

pafI-trnS-GGA

LSC · 52970-54355 · 1386 bp

Back to Corydalis

Pi 0.26
MarkerSeek score 76.80
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
1386
variable_sites
1151
pi
0.26
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 CCATTTATCCGTGATCTAGGCA GACCGCTCGGCCATCTCTC 45.5 68.4 57.7 62.5 318-1947 bp 0.918367 48.7
pafI-trnS-GGA_p2 CCATTTATCCGTGATCTAGGCA ACCGCTCGGCCATCTCTC 45.5 66.7 57.7 61.5 317-1946 bp 0.918367 48.7
pafI-trnS-GGA_p3 CCATTTATCCGTGATCTAGGCA GACCGCTCGGCCATCTCT 45.5 66.7 57.7 61.5 318-1947 bp 0.918367 48.7
pafI-trnS-GGA_p4 TCCATTTATCCGTGATCTAGGC GACCGCTCGGCCATCTCTC 45.5 68.4 57.5 62.5 319-1948 bp 0.918367 48.7
pafI-trnS-GGA_p5 TCCATTTATCCGTGATCTAGGC ACCGCTCGGCCATCTCTC 45.5 66.7 57.5 61.5 318-1947 bp 0.918367 48.7