Hotspot

psaA-pafI

LSC · 50304-50932 · 629 bp

Back to Corydalis

Pi 0.0513

MarkerSeek score 80.20

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 214
pi: 0.0513
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	ATGCCACTCAGCCAAAGAAA	GTTGAAGATCACGAGACGCT	45.0	50.0	58.0	58.0	819-936 bp	0.979592	91.1
psaA-pafI_p2	TGCCACTCAGCCAAAGAAAT	GTTGAAGATCACGAGACGCT	45.0	50.0	58.0	58.0	818-935 bp	0.979592	91.1
psaA-pafI_p3	GCTGGGTCCAATGTGAGTAG	GTTGAAGATCACGAGACGCT	55.0	50.0	58.0	58.0	889-1006 bp	0.959184	90.0
psaA-pafI_p4	ATGCCACTCAGCCAAAGAAA	AACCCACCTATTCTGACCGA	45.0	50.0	58.0	58.1	961-1078 bp	0.918367	88.3
psaA-pafI_p5	TGCCACTCAGCCAAAGAAAT	AACCCACCTATTCTGACCGA	45.0	50.0	58.0	58.1	960-1077 bp	0.918367	88.3