Hotspot

trnT-GGU-psbD

LSC · 40409-41309 · 901 bp

Back to Corydalis

Pi 0.0579

MarkerSeek score 80.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 331
pi: 0.0579
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCA	47.6	45.0	58.1	55.5	927-964 bp	0.979592	62.9
trnT-GGU-psbD_p2	TTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCA	45.0	45.0	56.6	55.5	926-963 bp	0.979592	60.1
trnT-GGU-psbD_p3	GTTCAAATCCGATAAGGGGC	TCTACCAAGGGCTATTGTCA	50.0	45.0	56.6	55.5	927-964 bp	0.979592	59.9
trnT-GGU-psbD_p4	GTTCAAATCCGATAAGGGGCT	ATCTACCAAGGGCTATTGTCA	47.6	42.9	58.1	56.1	928-965 bp	0.979592	59.6
trnT-GGU-psbD_p5	GTTCAAATCCGATAAGGGGCT	TCTACCAAGGGCTATTGTCAT	47.6	42.9	58.1	56.1	927-964 bp	0.979592	59.6